Finally, phylodynamic analysis substantiated the ongoing active expansion of USUV in Europe.Ĭonclusion: The data would imply a high potential for further USUV expansion in Europe. The results of this research revealed two newly described intercontinental migration events of USUV from Africa to Germany in the 1970s and from Africa to the Middle East (Israel) in the late 90s. Results: Herein, we report on a new USUV isolate from Culex pipiens collected in 2019 from Serbia. Methods: Phylogeographic and phylodynamic analyses were done using advanced phylogenetic methods implemented in Beast 1.10.4 and Beast 2.6.4 software packages. Detailed characterization of the pathogen's phylogenetics may offer valuable insights into the prediction and prevention of potential epidemics however, lack of uniformity and the number of available USUV sequences worldwide hamper comprehensive investigation.Īim: The study aimed to investigate USUV spatio-temporal dispersal inter- and intracontinentally and to estimate the dynamics of viral spread within Europe. Humans are accidental hosts and mostly develop mild or even asymptomatic infections, although severe complications such as encephalitis can also arise. 4Group for Medical Entomology, Centre of Excellence for Food- and Vector-Borne Zoonoses, National Institute of Republic of Serbia, Institute for Medical Research, University of Belgrade, Belgrade, Serbiaīackground: Usutu virus (USUV) is an emerging mosquito-borne Flavivirus, with birds as the main zoonotic reservoir.3Institute for Biocides and Medical Ecology, Belgrade, Serbia.2Department for Genetic Research, Institute for Biological Research “Sinisa Stankovic”, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia.1Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade, Belgrade, Serbia.Marina Siljic 1, Rastko Sehovic 1, Marko Jankovic 1, Gorana Stamenkovic 2, Ana Loncar 3, Marija Todorovic 1, Maja Stanojevic 1 and Valentina Cirkovic 4 *

2d drawing software